Topical Brazilian propolis improves corneal wound healing and inflammation in rats following alkali burns
نویسندگان
چکیده
BACKGROUND The objective of this study was to investigate the effects of the Brazilian Scaptotrigona sp propolis, a widely used folk medicine, in corneal wound healing and inflammation. METHODS Corneal epithelial defects of 1 mm in diameter were made in the right eyes of Wistar male adult rats by cauterization with silver nitrate sticks. Subsequently, they were divided in two groups (n = 40 rats/group): Brazilian propolis (BP) group was topically treated with a microemulsion containing 1% Brazilian propolis; vehicle (VH) group received the same formulation without propolis. The epithelial defect area was photographed and measured at t = 0 (wound induction), and after 12, 24, 48 and 120 h of treatment. The inflammatory response was evaluated based on counting of neutrophils. Epithelial regeneration rates were determined based on Ki-67 expression in basal epithelial cells. Comparisons were made using the Kruskal-Wallis and the Mann-Whitney U test. RESULTS The BP group presented both smaller epithelial defect areas at 12, 24 and 48 h and fewer corneal infiltrating neutrophils at 24 and 48 h (P < 0.01) than the VH group. These effects were associated with more pervasive Ki-67 staining in the BP group at 12 and 24 h (P < 0.05). CONCLUSIONS Topically applied BP accelerated wound healing and reduced the inflammatory response to silver nitrate-induced corneal alkali burns in rats.
منابع مشابه
Topical Green Propolis Improves Corneal Wound Healing and Inflammation in Rats following Alkaline Burns
Background: The objective of this study was to investigate the effects of green propolis, a widely used folk medicine, in corneal wound healing and inflammation. Methods: Epithelial defects of 1mm in diameter were made in the right corneas of Wistar male adult rats by cauterization with silver nitrate sticks. Subsequently, they were divided in two groups (n=40 rats/group): group green propolis ...
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Effects of topical chondrocyte-derived extracellular matrix treatment on corneal wound healing, following an alkali burn injury.
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Propolis is a resinous, sticky substance that bees collect from conifer buds and use to repair cracks in their hives. Propolis was used in folk medicine as early as 300 BC as an anti-inflammatory drug and wound healing agent. We studied the effect of Propolis on wound healing in albino rats. Twenty male albino rats had a linear 3 cm incision made over skin of back. The animals were randomly div...
متن کاملEffect of propolis on wound healing in albino rats
Propolis is a resinous, sticky substance that bees collect from conifer buds and use to repair cracks in their hives. Propolis was used in folk medicine as early as 300 BC as an anti-inflammatory drug and wound healing agent. We studied the effect of Propolis on wound healing in albino rats. Twenty male albino rats had a linear 3 cm incision made over skin of back. The animals were randomly div...
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